Table 1 PCR conditions and specific MgCl2 concentrations for the amplification of PAX2 transcripts and the housekeeping gene PBGD.
PCR conditions | ||||
|---|---|---|---|---|
target | MgCl2 (mmol/l) | Cycles | Temperature (°C) | Time (s) |
PBGD | 4 | 45 | 95 | 0 |
65 | 12 | |||
72 | 10 | |||
PAX2_1 | 1,5 | 55 | 95 | 0 |
55 | 10 | |||
72 | 8 | |||
PAX2_2 | 2 | 55 | 95 | 0 |
57 | 12 | |||
72 | 10 | |||