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Table 1 PCR conditions and specific MgCl2 concentrations for the amplification of PAX2 transcripts and the housekeeping gene PBGD.

From: An intron 9 containing splice variant of PAX2

PCR conditions
target MgCl2 (mmol/l) Cycles Temperature (°C) Time (s)
PBGD 4 45 95 0
    65 12
    72 10
PAX2_1 1,5 55 95 0
    55 10
    72 8
PAX2_2 2 55 95 0
    57 12
    72 10