Figure 3From: Bioactivity-guided identification and cell signaling technology to delineate the immunomodulatory effects of Panax ginseng on human promonocytic U937 cellsHigh performance liquid chromatography analysis of PGSE. The separation was done by using a reversed-phase column Lichrospher 100 C18 reversed-phase and the detection wavelength was set at 203 nm for all ginsenosides. The gradient program consisted of two solvents (A) water and (B) acetonitrile at a flow of 1 mL/min as follows: 0–6 min, 21–22% B; 6–7 min, 22–23% B; 7–25 min, 23–24% B; 25–30 min, 24–30% B; 30–40 min, 30–32% B; 40–45 min, 32–50% B; 45–60 min, 50–65% B; 60–61 min, 65–100% B; and 61–65 min, back to 21% B before the next injection for analysis. Twenty micrograms of PGSE was injected each time.Back to article page