Human embryonic stem cells hemangioblast express HLA-antigens

Table 1 The sequences of primers, product length and annealing temperatures used in RT-PCR reactions

Gene	Forward primer	Reverse primer	Size (bp)	Annealing temperature
β-Actin	TTTGAATGATGAGCCTTCGTCCCC	GGTCTCAAGTCAGTGTACAGGTAAGC	129	59
T	TGTCCCAGGTGGCTTACAGATGAA	GGTGTGCCAAAGTTGCCAATACAC	144	59
FOXA2	CCATTGCTGTTGTTGCAGGGAAGT	CACCGTGTCAAGATTGGGAATGCT	196	59
NeuroD	CCCATGGTGGGTTGTCATATATTCATGT	CCAGCATCACATCTCAAACAGCAC	196	59
KDR	CCTCTACTCCAGTAAACCTGATTGGG	TGTTCCCAGCATTTCACACTATGG	219	59
CD34	AAATCCTCTTCCTCTGAGGCTGGA	AAGAGGCAGCTGGTGATAAGGGTT	216	59
CD31	ATCATTTCTAGCGCATGGCCTGGT	ATTTGTGGAGGGCGAGGTCATAGA	159	59
SCL	AAGGGCACAGCATCTGTAGTCA	AAGTCTTCAGCAGAGGGTCACGTA	104	59
PTCH	CGCTGTCTTCCTTCTGAACC	ATCAGCACTCCCAGCAGAGT	282	60
GLI1	CTCTGAGACGCCATGTTCAA	ATCCGACAGAGGTGAGATGG	282	60
ε-globin	CACTAGCCTGTGGAGCAAGATGAA	AATCACCATCACGTTACCCAGGAG	304	59
γ-globin	CGCTTCTGGAACGTCTGAGGTTAT	CCAGGAGCTTGAAGTTCTCAGGAT	370	59
β-globin	TGTCCACTCCTGATGCTGTTATGG	AGCTTAGTGATACTTGTGGGCCAG	302	59

ISSN: 1479-5876