Figure 9

Assessment of AVOs formation by acridine orange staining. BA induces autophagy in ADF cells. A representative example of acridine orange stained cells. Bright orange granules are evident in BA and CsA+BA treated cells. As specificity control, cells treated with 200 nM bafilomycin A1 (BAF) for 30 minutes before the addition of acridine orange do not show AVOs formation.