Figure 4
Effect of CD437, LND, BA, CCCP and CsA treatment on mitochondrial transmembrane potential as assessed by JC1 staining. CD437, LND and BA treatment produces a dose dependent ΔΨ dissipation. (A) Vehicle treated cells stained with JC1. (B) ADF cells treated with BA at the ΔΨ IC50 dose and stained with JC1. Graphs indicate the dose dependent ΔΨ dissipation expressed as Red/green (R/G) fluorescence ratio. Each value has been normalized versus the R/G ratio of the vehicle treated control to which an arbitrary value of 100% has been assigned. Each point is the mean of two independent experiments performed in triplicate.