Increased susceptibility of viable ovarian cancer cells to immune attack after Doxil exposure. (A) Upregulation of MHC-I and Fas on ID8-E6E7 cells following treatment with Doxil at 0.1 μg/ml or 1 μg/ml. (B) Left, Increased sensitivity of ID8-E6E7 cells to CTL activated with IL-2 and E7 peptide. The effector to target ratio (E:T) is indicated. Each data point represents the mean of triplicate wells. Experiments repeated twice with similar results. (C) Treatment of ID8 cells with Doxil sensitizes them to Fas agonistic antibody (right). The ID8 cells (untreated or treated with Doxil) have been incubated with the Fas agonistic antibody and recombinant protein G or with isotype matched antibody and recombinant protein G for 24 hours. Cells were harvested (trypsin), stained with trypan blue and viable cells were counted. The bars show the means and standard error of the mean for three independent experiments.