Figure 2

Transduction efficiencies of CD34-pDC. CD34-pDC were non-transduced (NT) or transduced with E-GFP encoding vectors at day 6 after the induction of differentiation, then cultured for 6 additional days. pDC were gated in forward/side scatter, then analyzed for the expression of E-GFP by flow cytometry. (A) CD34⁺ progenitors were transduced by LV with a PGK promoter pseudotyped with either VSVG and RD114 envelopes at a MOI of 18 or with the GaLV envelope at a MOI of 9. (B) CD34⁺ progenitors were transduced with VSVG pseudotyped-LV with a PGK, EF1, desmin or C5–12 promoter, at a MOI of 18. (C) CD34⁺ progenitors were transduced by rAAV of serotype 1, 2 or 5 with a CMV promoter, with the number of viral genomes/cell indicated. Results are expressed as mean percentage of cell +/- SD over the number of independent experiments indicated.