Transduction efficiencies of GEN2.2. The pDC cell line, GEN2.2, was non-transduced (NT) or transduced with E-GFP encoding vectors then analysed 5 days posttransduction. GEN2.2 were gated in forward/side scatter, then analyzed for the expression E-GFP by flow cytometry. (A) GEN2.2 were transduced by LV with a PGK promoter pseudotyped with either VSVG and RD114 envelopes at a MOI of 18 or with the GaLV envelope at a MOI of 9. (B) GEN2.2 were transduced with VSVG pseudotyped-LV with a PGK, EF1, desmin or C5–12 promoter, at a MOI of 18. (C) GEN2.2 were transduced by rAAV of serotype 1, 2 or 5 with a CMV promoter, with the number of viral genomes/cell indicated. Results are expressed as mean percentage of cell +/- SD over the number of independent experiments indicated.