Figure 6
Immunoblot analysis of tumor tissue. (A) Representative images of EGFR/PI3K and STAT3 signaling pathways. EGFR, pEGFR, pSTAT3, STAT3, cyclin D1, Bcl-xL, survivin, p53, actin; (B) images for Bax, cleaved caspase-3, and PARP proteins. (C) Analysis of cleaved caspase-3 protein band showing an increase of cleaved caspase-3 in cisplatin-resistant tumor treated with aspirin (ASA) or NCX-4040 (5 mg/kg, daily), and NCX-4040 (5 mg/kg, daily) with cisplatin (cDDP, 8 mg/kg, single dose). (D) Analysis of PARP protein band showing an increase of PARP in cisplatin-resistant tumor treated with aspirin (ASA), NCX-4040, and NCX-4040 with cisplatin (cDDP). *p < 0.05 versus untreated (Control) group.