A highly invasive human glioblastoma pre-clinical model for testing therapeutics

Table 1 Metastatic potential of commonly used GBM cell lines.

Cell line	Mouse NO (n)	(+)	(++)	(+++)
U118	5	0	0	0
U251	5	0	1	1
U251-M1	5	0	2	3
U251-M2	8	0	1	7
U87	5	0	0	2
U87-M1	7	0	3	4
U87-M2	10	0	3	7
DBTRG-05MG*	7	1	5	1
DBM2*	7	0	3	4

^§To determine if invasive potential of GBM cells can be selected for in vivo, DBTRG 05MG, U251, U87 and U118 cells were subjected to experimental metastasis. 10⁶ cells in 100 μl PBS were injected through the tail vein of nude mice. Mice were sacrificed when they were moribund, and lungs with tumors were scored and transplanted as described in Materials and Methods.
*For the comparison between DBTRG-05MG and DBM2, mice were sacrificed 8 weeks after tumor inoculation.

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