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Table 9 IFNγ ELISPOT LOD

From: Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients

   

TIL1520

TIL1520

TIL1235

  

Peptide

gp100209

gp100pool

MART-1

PBMC High

TIL Cells/well

TIL/PBMC

   
 

80

1/5,000

53

57

15

 

40

1/10,000

27

30

14

 

20

1/20,000

15

17

4

 

8

1/50,000

3

6

0

 

4

1/100,000

6

0

1

PBMC Low

     
 

200

1/500

86

84

28

 

100

1/1000

52

59

19

 

50

1/2000

37

38

11

 

25

1/4000

22

31

7

 

12

1/8000

13

10

3

  1. TIL cells were spiked into 4 × 105 (High) or 105PBMC (Low) per well and limit of detection was determined (IFNγ Secreting Cell Frequency). The lowest IFNγ secreting cells detected above the background is considered LOD, values shown in bold. The corresponding TIL/PBMC ratio indicates assay sensitivity.
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Journal of Translational Medicine

ISSN: 1479-5876

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