Table 3 IFNγ real time RT-PCR accuracy and precision
Analysts 1 | Analysts 2 | |
|---|---|---|
Intra Assay (n = 80) | ||
Expected Value | 1000 | 1000 |
Detected Value (Mean) | 954 | 1233 |
SD | 109 | 216 |
Precision (% CV) | 11.4% | 17.5% |
Accuracy (% Recovery) | 95% | 123% |
Inter Assay (n = 18) | ||
Expected Value | 1000 | 1000 |
Detected Value (Mean) | 1100 | 1133 |
SD | 14 | 65 |
Precision (% CV) | 10.3% | 5.8% |
Accuracy (% Recovery) | 110% | 113% |