Characteristics of DC/Apo-Nec vaccine. A-DCs phagocytosis. Patient #1 results are shown. Apo-Nec cells (green labeled) phagocytosis by DCs (red-labeled, left panel) was evaluated as described under Methods. DCs and Apo-Nec cells were co-cultured for 48 hs at 37°C (middle panel) or at 4°C to inhibit phagocytosis (right panel). Total DCs (red labeled population) was gated and the percentage of double positive cells (DC/Apo-Nec) was calculated. B-DC Maturation. Markers expression of iDCs, DC/Apo Nec cells and DCs + LPS from all vaccinated patients (n = 15) was evaluated by FACS using monoclonal antibodies as described under methods. Results are mean ± SD percentage of positive cells, MFI: mean fluorescence intensities. C- DC endocytosis. FITC-Dx uptake of iDCs DC/Apo-Nec cells and DC + LPS of all vaccinated patients (n = 15). Results are indicated as mean ± SD percentage of FITC-Dx positive cells after 90 min incubation and washing. *: diferences with iDCs were statistically significant (Student's t test). D-DC migration. In vitro migration towards MIP-1α and MIP-3β was measured as described under methods. iDCs, DC/Apo-Nec and DCs + LPS from patient # 9 are shown (mean ± SD, assayed in triplicate).