Figure 5

LXR-623 treatment of human PBMC ex vivo significantly increases protein levels of ABCA1 and ABCG1. Peripheral blood mononuclear cells (PBMC) were purified from normal human donors (n = 3), transferred to cell culture dishes, and treated with vehicle (0.1% DMSO) or LXR-623 (2 uM) for either 24 or 48 hours. Following incubation, cells were lysed and protein extracts were separated on SDS-PAGE and blotted with antisera raised to ABCA1, ABCG1, or actin (to serve as an indicator of protein loading per lane). Horseradish peroxidase-linked secondary antibodies were bound to the immobilized protein/antibody complexes, and proteins were visualized by chemiluminescence. Duplicate lanes for each treatment reflect the two different donors analyzed in this experiment. Molecular masses were estimated by the relative mobility of protein markers run in an adjacent lane on each gel.