Figure 3

LXR-623 upregulates transcription of ABCA1 and ABCG1 in monkey whole blood cells proportional to dose. Cynomolgous monkeys maintained on normal chow were orally dosed with 0, 15 and 50 mpk/day of LXR-623 for 7 days (n = 4 per dose group). Blood was collected on day 7 of dosing, and RNA was prepared from whole blood for gene expression analysis of ABCA1 and ABCG1. qPCR was performed using assays designed to measure monkey (A) ABCA1 and (B) ABCG1 transcripts, and the measured amounts of these transcripts were normalized to monkey 18S RNA levels in each sample. Bars indicate the mean fold change of normalized ABCA1 or ABCG1 transcript levels +/- SEM in the indicated dose group compared to vehicle treated animals at the same time point. *p < 0.05, **p < 0.01 compared to vehicle treatment, as determined by Student's t test.