Figure 4
Cytotoxicity assay. Multiple AAV vectors for DC loading and the autologous targets generated using the IE1 sub-genes. Targets were generated by viral loading of the IE1 sub-genes into PBMC. Resulting CTL killing is shown. Note that T cells stimulated by mock-infected (no Ag) loaded DCs, AAV only-loaded DCs AAV/GFP-loaded DCs AAV/E6-loaded DCs did not kill IE1-positive targets. However, T cells stimulated by AAV/GFP/IE1-loaded DCs did kill IE1-positive target cells. These data strongly suggest high antigen-loading specificity of the CTLs generated by AAV/GFP/IE1 infection of DCs.