Figure 3

IE1 expression in infected DCs. Total RNA was isolated from mock-infected and AAV/IE1-infected adherent monocytes at 72 hours after infection. These samples were analyzed by RT-PCR and PCR, as indicated, for the presence of IE1 RNA. PCR product resulting from using the AAV/IE1 vector plasmids as templates was the positive controls. RT-PCR analysis for the cellular TFIIB mRNA was considered as further control. Note that only cDNA from cells infected with AAV/IE1 virus resulted in an appropriate RT-PCR sized product, whereas mock-infected cells did not.