Figure 4

Histological examination of the vasculature in the non-ischemic and ischemic striatum on day 28 after MCAo. A-C: Immunostaining of mouse PECAM-1 (red)/Neu-N (blue) in the non-ischemic striatum (A), and the ischemic striatum in saline (B)-and hMNC (C)-injected mice. Arrows show human PECAM-1⁺ (green) cells in the ischemic striatum in the hMNC-injected group. D-E: Representative fluorescent photographs of the ischemic striatum stained for mouse PECAM-1 (blue), Neu-N (green) and CM-DiI (red) in hES-EC-injected mice. F-G: Immunostaining of αSMA (blue)/mouse PECAM-1 (green)/human HLA-A,B,C (red) in the ischemic striatum in the hES-MC-injected mice. Human HLA positive and αSMA positive hES-MCs were shown as purple (red+blue) cells. H, Immunostaining of mouse PECAM-1 (red)/Neu-N (blue)/human Pecam-1 (green) in the ischemic striatum in the hES-EC+MC-injected groups. I, Localization of transplanted hES-ECs+MCs in the ischemic striatum stained for αSMA (blue)/mouse PECAM-1 (green)/human HLA-A,B,C (red). A-D/F/H, scale bar: 100 μm, ×20 magnification. E/G/I, scale bar: 20 μm, ×63 magnification.