Table 1 Candidate biomarkers for human cancers discovered by cancer secretome analysis

Lung

SDS-PAGE/nano-ESI-MS/MS

ELISA

CD98, fascin, 14-3-3 η, polymeric immunoglobulin receptor/secretory component

[73]

2-DE/MALDI-TOF/TOF-MS

Western blot/ELISA/IHC

Cathepsin D

[28]

2-DE/MALDI-TOF-MS

RT-PCR/western blot/ELISA/IHC

Dihydrodiol dehydrogenase

[27]

SDS-PAGE/MALDI-TOF-MS

ELISA

L-lactate dehydrogenase B

[90]

2-DE/MALDI-TOF-MS

RT-PCR/enzyme activity detection

Mn-SOD

[29]

Liver

LC-MS/MS

Western blot

Apolipoprotein E, DJ-1, apolipoprotein H, galectin-3, cathepsin L, cyclophilin A, cystatin C

[41]

Pancreatic

NuPAGE/LC-MS/MS/SILAC

Western blot/IHC

CD9, perlecan, SDF4, apolipoprotein E, fibronectin receptor, Mac-2 binding protein, cathepsin D, cathepsin B, MCP-1, L1CAM

[25]

LC-MS/MS

RT-PCR/western blot/IHC

CSPG2/versican, Mac25/angiomodulin

[43]

Bladder

SDS-PAGE/MALDI-TOF-MS

Western blot

Pro-u-plasminogen activator

[91]

LC-MS/MS

CXCL1

[92]

Nasopharyngeal

SDS-PAGE/MALDI-TOF-MS

Western blot/ELISA/IHC

Fibronectin, Mac-2 binding protein, plasminogen activator inhibitor 1

[69]

Prostate

LC-MS/MS

Western blot/ELISA

Mac-2 binding protein

[40]

Oligonucleotide microarray/genome-based computational prediction

RT-PCR/ELISA/IHC

Macrophage inhibitory cytokine 1

[22]

LC-MS/MS

ELISA

follistatin, chemokine (C-X-C motif) ligand 16, pentraxin 3, spondin 2

[93]

Melanoma

NuPAGE/LC-Q-TOF-MS/MS

Western blot

Cathepsin D, gp100

[79]

Breast

LC-MS/MS

Western blot

Galectin-3-binding protein, alpha-1-antichymotrypsin

[94]

LC-MS/MS

ELISA

Elafin

[95]

Colorectal

SDS-PAGE/MALDI-TOF-MS

Q-PCR/Western blot/IHC/ELISA

Collapsing response mediator protein-2

[72]

2-DE/DIGE/MALDI-TOF-MS

Northern blot/western blot

Cathepsin D, stratifin, calumenin

[21]

Renal

2-DE/MALDI-TOF-MS/immunoblotting

Western blot/homogeneous fluorescent immunoassay

Pro-MMP-7

[68]

Oral

SDS-PAGE/MALDI-TOF-MS

Western blot/IHC/ELISA

Mac-2 binding protein

[70]

Fibrosarcoma

Capillary ultrafiltration probe/2-DE/MALDI-TOF-MS

Cyclophilin A, S100A4, profiling-1, thymosin beta 4, thymosin beta 10, fetuin-A, alpha-1 antitrypsin 1–6, contrapsin, apolipoprotein A-1, apolipoprotein C-1

[31]

Ovarian

SELDI-TOF MS

Immunodepletion

CXC chemokine ligand 1, intact and truncated interleukin 8

[66]

HPLC fractionation/LC-MS/MS

Immunoblot/immunofluorescence

14-3-3 zeta

[96]