Figure 6

The ability of vaccination with autologous FCs to stimulate T cells. A, Nonadherent PBMCs obtained before and after vaccination were stimulated with unirradiated DCs/auto-HCC, DCs, auto-HCC cells, or an unfused mixture of both for 7 days. T-cell proliferation assay was performed by Cell Titer 96 Nonradioactive Cell Proliferation Assay Kit according to the protocol. B, Nonadherent PBMCs obtained before and after vaccination were stimulated with unirradiated DCs/auto-HCC and examined under a microscope (magnification, ×10). Similar results were obtained in three individual experiments. C, Nonadherent PBMCs obtained before (left panel) and after vaccination (right panel) were stimulated with unirradiated DCs/auto-HCC and stained with FITC-conjugated anti-CD4 and PE-conjugated anti-CD8 mAb. Numbers represent cells positively staining for the indicated surface markers. D, Nonadherent PBMCs obtained before (left panel) and after vaccination (right panel) were stimulated with unirradiated DCs/auto-HCC. Percentage of IFN-γ-positive CD4⁺ or CD8⁺ T cells was assessed. For each group, the mean ± SD of three experiments is shown.