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Figure 5 | Journal of Translational Medicine

Figure 5

From: In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells

Figure 5

Phenotypic characterization of DCs/auto-HCC. A, Autologous HCC cells (auto-HCC) and DCs generated before and after vaccination were analyzed by flow cytometry for expression of the indicated antigens. Thin line, isotype control; thick line, indicated antigens. Numerical values show the MFIs of indicated antigens in DCs. B, MFIs of HLA-ABC, HLA-DR, CD80, CD86, and CD83 in DCs generated before and after vaccination were analyzed. C, Fusions of auto-HCC and DCs (before or after vaccination) were analyzed by 2-color flow cytometry for dual expression of WT1 and HLA-DR/CD86. Numbers represent cells positively staining for the indicated surface markers.

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