Figure 2
Phenotypic analysis of DCs/allo-HCC fusion cells created in the presence of HCCsp. A, Four types of DC were analyzed by flow cytometry for expression of the indicated antigens (tinted area) B, Four types of FC preparation 1) DCs/allo-HCC; 2) DCs/allo-HCC/sp; 3) OK-DCs/allo-HCC; and 4) OK-DCs/allo-HCC/sp were analyzed by two-color flow cytometry for expression of WT1 and HLA-DR. Numbers represent cells positively staining for the indicated surface markers. C, Percentage of cells positive for WT1 and HLA-DR in four types of FC preparation from three healthy donors was analyzed. For each group, the mean ± SD is shown. *, Significant differences.