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Table 3 CCL21 production.

From: Pre-clinical characterization of GMP grade CCL21-gene modified dendritic cells for application in a phase I trial in Non-Small Cell Lung Cancer

CCL21 in DC supernatant
[ng]/cell number
24 h 48 h 72 h
DC CCL21-DC CV-DC DC CCL21-DC CV-DC DC CCL21-DC
Cell number: ×106 Cell number: ×106
1 1 2.5 5 10 1 1 1 2.5 5 10 1 1 4
n = 16 n = 16 n = 9 n = 6 n = 2 n = 6 n = 12 n = 10 n = 7 n = 7 n = 2 n = 6 n = 4 n = 4
ng/cell number ng/cell number ng/cell number
*0.0 2.3 ± 1.9 3.8 ± 2.8 3.5 ± 3.4 7.5 ± 2.4 *0.0 *0.0 3.7 ± 2.4 5.2 ± 2.5 4.4 ± 2.9 8.7 ± 1.1 *0.0 *0.0 6.7 ± 1.5
  1. * Below detection
  2. The capacity of DC to secrete CCL21 was evaluated by ELISA. Mock-transduced DC (DC), CV-DC and CCL21-DC were cultured at the concentrations indicated in 1 ml culture medium. The supernatant was analyzed after 24, 48, and 72 h cultures. The concentration of CCL21 is expressed in ng per cell number. The values represent the average ± SD of different independent experiments, as indicated.