Pre-clinical characterization of GMP grade CCL21-gene modified dendritic cells for application in a phase I trial in Non-Small Cell Lung Cancer

Table 3 CCL21 production.

CCL21 in DC supernatant [ng]/cell number
24 h						48 h						72 h
DC	CCL21-DC				CV-DC	DC	CCL21-DC				CV-DC	DC	CCL21-DC
Cell number: ×10⁶						Cell number: ×10⁶
1	1	2.5	5	10	1	1	1	2.5	5	10	1	1	4
n = 16	n = 16	n = 9	n = 6	n = 2	n = 6	n = 12	n = 10	n = 7	n = 7	n = 2	n = 6	n = 4	n = 4
ng/cell number						ng/cell number						ng/cell number
*0.0	2.3 ± 1.9	3.8 ± 2.8	3.5 ± 3.4	7.5 ± 2.4	*0.0	*0.0	3.7 ± 2.4	5.2 ± 2.5	4.4 ± 2.9	8.7 ± 1.1	*0.0	*0.0	6.7 ± 1.5

* Below detection
The capacity of DC to secrete CCL21 was evaluated by ELISA. Mock-transduced DC (DC), CV-DC and CCL21-DC were cultured at the concentrations indicated in 1 ml culture medium. The supernatant was analyzed after 24, 48, and 72 h cultures. The concentration of CCL21 is expressed in ng per cell number. The values represent the average ± SD of different independent experiments, as indicated.

ISSN: 1479-5876