Table 2 Primers/probes sets for quantification of PBGD and Cα-chain (FRET).
Primer/probe | Sequence (5'-3') | TA | MgCl2 (mM) | Standard dilutions (pg/μl) |
|---|---|---|---|---|
PBGD-fwd | TGC AGG CTA CCA TCC ATG TCC CTG C | 65°C | 4 | 1/0.1/0.001 |
PBGD rev | AGC TGC CGT GCA ACA TCC AGG ATG T | |||
PBGD-3Fl | CGT GGA ATG TTA CGA GCA GCA GTG ATG CCT ACC-Fl | |||
PBGD-5LC | LC-TGT GGG TCA TCC TCA GGG CCA TCT TC-ph | |||
HAC2-fwd | ACA CCT TCT TCC CCA G | 60°C | 3 | 10/1/0.01 |
HAC2-rev | TCC AGT TGG TGG CAT T | |||
HAC2-3Fl | GTG ATT GGG TTC CGA ATC CTC C-Fl | |||
HAC2-5LC | LC-CTG AAA GTG GCC GGG TTT AAT CT-ph |