Figure 1

Primers and probe annealing sites and Vβ-families distribution in peripheral blood of healthy volunteers. (A) Structure of the β-chain and annealing sites of primers and of the TaqMan probe. Forward primers (HBV) anneal at the variable region, reverse primer (HBC) and TaqMan probe anneal at the constant region (B) Average percentages and standard deviations for Vβ-families in PBMCs obtained from 20 healthy controls. Family percentages Pj [%] reflecting single family expression by the sum of all measured Vβ-families are shown.