Figure 3
Immunoblot of PSC cell culture supernatants and cell lysates. Cell culture supernatants and cell lysates of vehicle-, TGFbeta-, LPS- and TNFalpha-treated PSC were subjected to SDS-PAGE followed by incubation with (A; supernatants) anti-collagen1 and anti-fibronectin antibodies or (B; cell lysates) an anti-gamma-tubulin antibody Densitometry is shown in percent of vehicle-treated control PSC supernatants; n.s. = statistically not significant.