Table 1 Panel of antibodies used in this study
Antibody | Clone | Antigen retrieval | Dilution | Main cell reactivity | Source |
|---|---|---|---|---|---|
CD68 | PGM1 | Citrate | 1:50 | Macrophages | Dako, Glostrup, Denmark |
CD163 | 10D6 | Citrate | 1:100 | Macrophages | Novocastra, Newcastle upon Tyne, UK |
S100 | rabbit polyclonal | Citrate | 1:500 | DC | Dako |
CD11c | 5D1 | Citrate | 1:50 | interdigitating DC, stromal DC | Novocastra |
DC-LAMP/CD208 | 104.G4 | Protease | 1:50 | mature DC | Immunotech, Marseille Cedex, France |
DC-SIGN/CD209 | DCN46 | Citrate | 1:100 | stromal DC | BD Pharmingen, San Diego, CA, USA |
CD123 | 9F5 | Citrate | 1:10 | plasmacytoid DC | BD Pharmingen |
CD1a | 010 | Citrate | 1:50 | Langerhans cells, interdigitating DC | Dako |
Langerin/CD207 | 12D6 | Citrate | 1:100 | Langerhans cells | Novocastra |