Figure 6

DNA oxidative damage. After (A) a 6-hour exposure to 10 μM of NCX 4040 in LoVo and (B) a 14-hour exposure to 50 μM of NCX 4040 in LRWZ, cells were exposed to the antibody that specifically recognizes the presence of nuclear 8-hydroxyguanine lesions, which are almost exclusively elicited by oxidative stress. Each 100× magnification shows a detail of the corresponding 40× photo. All the pictures are representative of three independent experiments.