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Table 1 In vivo compound treatments used in training and testing

From: Diagnosis of drug-induced renal tubular toxicity using global gene expression profiles

Compound Class Conducted by Dose (mpk) Necropsy day Vehicle – Route
Cisplatin DNA – alkylator Merck 0.5 3, 8 0.9% (w/v) sodium chloride – IP
    3.5 3, 8  
    7 3, 8  
Cyclosporin A Calcineurin inhibitor Merck 6 3, 9, 15 olive oil – SC
    30 3, 9, 15  
    60 3, 9, 15  
Gentamycin Antibiotic Merck 20 3, 9, 15 0.9% (w/v) sodium chloride – IP
    80 3, 9, 15  
    240 3, 9, 12  
Sodium Fluoride Environmental toxin Merck 35 3, 8, 12 Water – PO
    75 3, 8, 12  
Merck X Antibiotic Merck 75 3, 8, 14 0.5%saline – IV
    150 3, 8, 14  
    225 3, 8  
Allopurinol Xanthine oxidase inhibitor Charles River 6 3 corn oil – IP
    30 3  
    100 3, 7, 14  
D-serine Serine analog Charles River 750 3, 14 water – IP
Hexachloro 1,3, butadiene Synthetic toxin Charles River 7.5 3 corn oil – IP
    40 3, 14  
    100 3  
Puromycin Antibiotic Charles River 5 3 0.9% (w/v) sodium chloride – IP
    20 3, 7, 14  
    60 3, 7  
Tobramycin Antibiotic Charles River 6 3 0.9% (w/v) sodium chloride – IP
    30 14  
    60 3, 14  
  1. Male Sprague-Dawley rats were treated daily with the listed compounds except D-serine which was given as a single dose once on day 0. Each dose group includes 4 or 5 rats. Animals were terminated at the end of study. Terminal or interim necropsy were performed 24 hours post dosing. Kidney expression profiles were obtained for each necropsy day (when kidney samples were harvested). Appropriate dosing routes were applied: PO – Oral Garvage, IV – intravenous, SC – subcutaneous.