Absence of a relationship between immunophenotypic and colony enumeration analysis of endothelial progenitor cells in clinical haematopoietic cell sources

Table 1 Percentage of cells expressing CD34 and/or CD133 in haematopoietic stem cell clinical transplant sources and normal blood.

Sources	BM	CB	mPBP	nPB
total leucocytes/ml (10⁶)	1.63 (0.3) n = 10	1.54 (0.4) n = 21	1.41 (0.26) n = 27	1.17 (0.41) n = 16
CD34+ (% of wbc)	0.71 (0.1) n = 10	0.42 (0.04) n = 21	1.46 (0.36) n = 27	0.09 (0.02) n = 16
CD133+ (% of wbc)	0.46 (0.07) n = 8	0.4 (0.05) n = 7	1.04 (0.2) n = 7	0.07 (0.01) n = 16
CD133+ (% of CD34+)	13 (5.65) n = 8	53 (6.14) n = 7	79.76 (5.5) n = 7	11 (3.96) n = 16

Percentages of cells expressing CD34 and/or CD133 alone or together were compared between the various sources tested. Normal (non-mobilised) peripheral blood (nPB), bone marrow (BM), umbilical cord blood (CB) and G-CSF-mobilised peripheral blood samples (mPBP). Results are expressed as the mean (standard error); n = number of samples tested. White blood cells (wbc) are all leucocytes in the samples analysed, including mononuclear and granulocytic populations.

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