Figure 4

Ex vivo intracellular IFN-γ staining of splenocytes from unimmunized mice or mice immunized against E7: 11–20, E7: 86–93 or E6: 29–38 alone. Splenocytes not stimulated with exogenous peptide produced background levels of IFN-γ staining splenocytes (panel A). as did splenocytes from immunized with E6: 29–38 (panel B) and E7: 86–93 (panel C) Only immunization against E7: 11–20 (panel D) caused expansion of CD8 ⁺/IFN-γ ⁺T-cells above background levels.