Figure 3

Ex vivo intracellular IFN-γ staining of splenocytes from mice immunized against E7: 82–90 alone or a mixture of four peptides consisting of E7: 11–20, E7: 82–92, E7: 86–93 and E6: 29–38. Splenocytes not stimulated with exogenous peptide produced background levels of IFN-γ staining splenocytes (panel A). Only immunization against E7: 82–90 (panel B) or a mixture of the four peptides (panel C) caused expansion of CD8 ⁺/IFN-γ ⁺ T-cells above background levels.