Table 2 Characteristics of primary DCs following maturation with different cocktails
From: Generation of clinical grade dendritic cells with capacity to produce biologically active IL-12p70
Parameter | Â | DC1 | DC2 | DC3 | DC4 | DC5 |
|---|---|---|---|---|---|---|
Recovery (% seeded cells) | N* | 4 | 4 | 3 | 2 | 2 |
| Â | m+ | 32.8 | 17.6 | 19.0 | 17.9 | 18.1 |
| Â | std# | 18.7 | 9.2 | 5.5 | 3.0 | 6.0 |
Recovery (% CD14+cells) | N* | 4 | 4 | 3 | 3 | 2 |
| Â | m+ | 42.6 | 22.2 | 25.4 | 24.0 | 25.5 |
| Â | std# | 22.0 | 9.7 | 6.2 | 2.8 | 3.1 |
% viable cells (7AAD-neg cells) | N* | 5 | 5 | 4 | 5 | 3 |
| Â | m+ | 97.5 | 88.9 | 96.0 | 96.0 | 94.0 |
| Â | std# | 0.9 | 3.7 | 1.6 | 1.2 | 1.4 |
% CD14+cells | N* | 5 | 5 | 4 | 5 | 3 |
| Â | m+ | 2.2 | 2.5 | 1.6 | 2.5 | 3.1 |
| Â | std# | 2.2 | 3.0 | 0.9 | 3.1 | 4.1 |
% CD83+ cells | N* | 5 | 5 | 4 | 5 | 3 |
| Â | m+ | 94.2 | 82.3 | 95.1 | 90.6 | 92.4 |
| Â | std# | 3.0 | 7.1 | 2.3 | 10.7 | 1.7 |
% CCR7+ cells | N* | 5 | 5 | 4 | 5 | 3 |
| Â | m+ | 79.7 | 67.5 | 75.2 | 74.4 | 85.2 |
| Â | std# | 7.2 | 5.6 | 8.7 | 11.8 | 0.7 |
% CD80+ cells | N* | 5 | 5 | 4 | 5 | 3 |
| Â | m+ | 80.7 | 70.8 | 83.2 | 85.5 | 85.3 |
| Â | std# | 12.5 | 10.9 | 10.7 | 8.9 | 11.0 |
% CD86+ cells | N* | 5 | 5 | 4 | 5 | 3 |
| Â | m+ | 95.1 | 89.6 | 94.2 | 94.7 | 95.8 |
| Â | std# | 2.2 | 4.5 | 2.2 | 2.1 | 0.8 |