Generation of clinical grade dendritic cells with capacity to produce biologically active IL-12p70

Table 1 Cocktail compositions used for DC maturation

DC population	Cocktail^a	Inflammatory cytokines/interferons	Other additives	TRL-ligands	Comments
DC1	Jonuleit	TNF, IL-1β, IL-6	PGE2	-	no TLR ligands
DC2	Kalinski	TNF, IL-1β, IFNα, IFNγ	-	poly (I:C)	TLR3/non-TLR ligand
DC3	Cocktail 3^b	TNF, IL-1β, IFNγ	PGE2	R848	TLR8 ligand, less IFNγ and PGE2
DC4	Cocktail 4^b	TNF, IL-1β, IFNγ	PGE2	R848	TLR8 ligand
DC5	Cocktail 5^b	TNF, IL-1β, IFNγ	PGE2	R848, poly (I:C)	TLR3/non-TLR, TLR8 ligands

^a The following concentrations were used for the individual cocktails: Jonuleit: 10 ng/ml TNF, 10 ng/ml IL-1β, 15 ng/ml IL-6 and 1 μg/ml prostaglandin E2; Kalinski: 10 ng/ml TNF, 10 ng/ml IL-1β, 3000 IU/ml IFNα, 1000 IU/ml IFNγ and 20 ng/ml poly (I:C); Cocktail 3: 10 ng/ml TNF, 10 ng/ml IL-1β, 1000 IU/ml IFNγ, 1 μg/ml R848 and 100 ng/ml prostaglandin E2; Cocktail 4: 10 ng/ml TNF, 10 ng/ml IL-1β, 5000 IU/ml IFNγ, 1 μg/ml R848 and 250 ng/ml prostaglandin E2; Cocktail 5: 10 ng/ml TNF, 10 ng/ml IL-1β, 5000 IU/ml IFNγ, 1 μg/ml R848, 250 ng/ml prostaglandin E2 and 20 ng/ml poly (I:C).
^b These represent compositions first reported here.

ISSN: 1479-5876