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Table 1 Cocktail compositions used for DC maturation

From: Generation of clinical grade dendritic cells with capacity to produce biologically active IL-12p70

DC population Cocktaila Inflammatory cytokines/interferons Other additives TRL-ligands Comments
DC1 Jonuleit TNF, IL-1β, IL-6 PGE2 - no TLR ligands
DC2 Kalinski TNF, IL-1β, IFNα, IFNγ - poly (I:C) TLR3/non-TLR ligand
DC3 Cocktail 3b TNF, IL-1β, IFNγ PGE2 R848 TLR8 ligand, less IFNγ and PGE2
DC4 Cocktail 4b TNF, IL-1β, IFNγ PGE2 R848 TLR8 ligand
DC5 Cocktail 5b TNF, IL-1β, IFNγ PGE2 R848, poly (I:C) TLR3/non-TLR, TLR8 ligands
  1. a The following concentrations were used for the individual cocktails: Jonuleit: 10 ng/ml TNF, 10 ng/ml IL-1β, 15 ng/ml IL-6 and 1 μg/ml prostaglandin E2; Kalinski: 10 ng/ml TNF, 10 ng/ml IL-1β, 3000 IU/ml IFNα, 1000 IU/ml IFNγ and 20 ng/ml poly (I:C); Cocktail 3: 10 ng/ml TNF, 10 ng/ml IL-1β, 1000 IU/ml IFNγ, 1 μg/ml R848 and 100 ng/ml prostaglandin E2; Cocktail 4: 10 ng/ml TNF, 10 ng/ml IL-1β, 5000 IU/ml IFNγ, 1 μg/ml R848 and 250 ng/ml prostaglandin E2; Cocktail 5: 10 ng/ml TNF, 10 ng/ml IL-1β, 5000 IU/ml IFNγ, 1 μg/ml R848, 250 ng/ml prostaglandin E2 and 20 ng/ml poly (I:C).
  2. b These represent compositions first reported here.