Figure 5

Mechanisms of DC-mediated tumor protection. (a) Humoral anti-Id responses in mice vaccinated with DC that were pulsed with the purified A20 Id/anti-FcγR heterodimeric BiV protein or with whole BiV cells. Results were expressed as those reciprocal Ab titres that gave two-fold extinction above background. Means were calculated from 6 (BiV protein) and from 12 mice (BiV cells), respectively. Standard deviations are also indicated. (b) Effect of CD4⁺ and CD8⁺ T-cell depletion on survival of mice treated with DC that were pulsed with BiV cells. The survival of CD4⁺ or CD8⁺ T-cell-depleted mice was not significantly different from the tumor control group that did not receive any therapy (logrank test). (c) Survival of tumor-bearing mice that were injected with trioma cell-loaded DC from β₂m-deficient animals which were or were not matured by LPS following the loading step. The therapeutic effect of BiV cell-pulsed mDC from β₂m-deficient animals was not significantly different from BiV cell-pulsed mDC from normal mice (logrank test). Tumor control groups without therapy are also shown in both survival diagrams. Typical results are depicted from two experiments in each setting.