Figure 4

Effect of recombinant LT-α1β2 treatment on D5 tumor cells. (A) Macrophage chemotactic activity of D5 supernatant after treatment with LT-α1β2. Supernatant derived from cultured D5 tumor cells or D5 tumor cells that were treated with recombinant LT-α1β2 were plated in the lower chamber of a transwell plate. The number of DJ2P macrophage cells placed on the upper chambers that trans-migrated into the lower chamber was determined by FACS analysis with FITC-labeled antiCD11b antibody. (B) Chemokine expression by D5 tumor cells induced by LT-α1β2. Expression of chemokines by D5 tumor cells before or after LT-α1β2 treatment were analyzed by RT-PCR. HPRT expression was used for the control of total mRNA. Production of MCP-1 (C) and KC (D) and by D5 tumor cells in the supernatant after LT-α1β2 treatment was measured by ELISA. The error bars represents standard error of 2–3 experiments.