Table 1 Panel A: List of the biologically active factors released by activated platelets. Panel B: Protocol for the preparation of PRP in each bioassay. The amount of blood collected, the centrifugal force, and the final PRP volume are shown.
Panel A | |||||
|---|---|---|---|---|---|
Biologically active factors released by the activated platelets11–15 | |||||
ADP, ATP, Serotonin, Platelet-derived Growth Factor (PDGF), Transforming Growth Factor-β1 (TGF-β1), Connective Tissue Activating Peptide III (CATP III), Insuline-like Growth Factor-1 (IGF-1), Epidermal Growth Factor (EGF), Vascular Endothelial Growth Factor (VEGF), basic Fibroblast Growth Factor (bFGF), Angiopoietin-2 (Ang-2), Thrombospondin, Factor V, Factor XI, Factor XIII, Platelet-derived endothelial cell growth factor (PDECGF), Osteocalcin, and adhesive proteins such as Fibrinogen, Fibronectin, von Willebrand Factor (vWF), and P-Selectin. | |||||
Panel B | |||||
Bioassay | Collected Blood (final volume, including anticoagulant) | Collection site | First spinning (RCF) | Second spinning (RCF) | PRP volume |
Heterotopic bioassay (Ferret) | 5 ml | Jugular vein | 40 g for 20' | 235 g for 10' | 250 μl |
Orthotopic bioassay (Rat) | 3 ml | Jugular vein | 380 g for 2'15" | 380 g for 5' | 75 μl |
Orthotopic bioassay (Non-human primate) | 30 ml | Cephalic vein | 1130 g for 2'15" | 1130 g for 5' | 1.5 ml |
Clinical cases ( Homo sapiens ) | 20 ml | Cephalic vein Basilic vein Median cubital vein | 1130 g for 2'15" | 1130 g for 5' | 1 ml |