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Table 1 Panel A: List of the biologically active factors released by activated platelets. Panel B: Protocol for the preparation of PRP in each bioassay. The amount of blood collected, the centrifugal force, and the final PRP volume are shown.

From: Calcium Sulfate and Platelet-Rich Plasma make a novel osteoinductive biomaterial for bone regeneration

Panel A
Biologically active factors released by the activated platelets11–15
ADP, ATP, Serotonin, Platelet-derived Growth Factor (PDGF), Transforming Growth Factor-β1 (TGF-β1), Connective Tissue Activating Peptide III (CATP III), Insuline-like Growth Factor-1 (IGF-1), Epidermal Growth Factor (EGF), Vascular Endothelial Growth Factor (VEGF), basic Fibroblast Growth Factor (bFGF), Angiopoietin-2 (Ang-2), Thrombospondin, Factor V, Factor XI, Factor XIII, Platelet-derived endothelial cell growth factor (PDECGF), Osteocalcin, and adhesive proteins such as Fibrinogen, Fibronectin, von Willebrand Factor (vWF), and P-Selectin.
Panel B
Bioassay Collected Blood (final volume, including anticoagulant) Collection site First spinning (RCF) Second spinning (RCF) PRP volume
Heterotopic bioassay (Ferret) 5 ml Jugular vein 40 g for 20' 235 g for 10' 250 μl
Orthotopic bioassay (Rat) 3 ml Jugular vein 380 g for 2'15" 380 g for 5' 75 μl
Orthotopic bioassay (Non-human primate) 30 ml Cephalic vein 1130 g for 2'15" 1130 g for 5' 1.5 ml
Clinical cases ( Homo sapiens ) 20 ml Cephalic vein
Basilic vein
Median cubital vein
1130 g for 2'15" 1130 g for 5' 1 ml