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Table 1 Panel A: List of the biologically active factors released by activated platelets. Panel B: Protocol for the preparation of PRP in each bioassay. The amount of blood collected, the centrifugal force, and the final PRP volume are shown.

From: Calcium Sulfate and Platelet-Rich Plasma make a novel osteoinductive biomaterial for bone regeneration

Panel A

Biologically active factors released by the activated platelets11–15

ADP, ATP, Serotonin, Platelet-derived Growth Factor (PDGF), Transforming Growth Factor-β1 (TGF-β1), Connective Tissue Activating Peptide III (CATP III), Insuline-like Growth Factor-1 (IGF-1), Epidermal Growth Factor (EGF), Vascular Endothelial Growth Factor (VEGF), basic Fibroblast Growth Factor (bFGF), Angiopoietin-2 (Ang-2), Thrombospondin, Factor V, Factor XI, Factor XIII, Platelet-derived endothelial cell growth factor (PDECGF), Osteocalcin, and adhesive proteins such as Fibrinogen, Fibronectin, von Willebrand Factor (vWF), and P-Selectin.

Panel B


Collected Blood (final volume, including anticoagulant)

Collection site

First spinning (RCF)

Second spinning (RCF)

PRP volume

Heterotopic bioassay (Ferret)

5 ml

Jugular vein

40 g for 20'

235 g for 10'

250 μl

Orthotopic bioassay (Rat)

3 ml

Jugular vein

380 g for 2'15"

380 g for 5'

75 μl

Orthotopic bioassay (Non-human primate)

30 ml

Cephalic vein

1130 g for 2'15"

1130 g for 5'

1.5 ml

Clinical cases ( Homo sapiens )

20 ml

Cephalic vein

Basilic vein

Median cubital vein

1130 g for 2'15"

1130 g for 5'

1 ml