Figure 3

The intensity of c-mpl expression can define the most primitive HSC. CD34-FITC vs. CD38-PE smoothed pseudocolor dot plots derived from the same representative human CD34⁺ ABM and mPBSC specimens as depicted in Fig. 1A. From each specimen, all cell events were initially categorized by their percentile level of fluorescence on the c-mpl-APC parameter on a log histogram plot. Five gates were drawn on the histogram plot to define a progression of five discrete levels of High intensity c-mpl expression: 79–80, 84–85, 89–90, 94–95, and 99–100 percentiles. Viable cell events falling within each percentile range are depicted on the corresponding smoothed dot plot.