Patient ascites spheroids #6–10 disaggregate on mesothelial cell monolayers. Patient ascites spheroids were added to confluent mesothelial monolayers for 7 days to determine their invasive potential. Photos show representative examples of patient samples 6, 8, 9, and 10 at t = 0, and days 1, 4, and 7. Arrows delineate the perimeters of disaggregating spheroids. Magnification = 100×.