Patient ascites spheroids #2–5 disaggregate on or invade mesothelial cell monolayers. Patient ascites spheroids were added to confluent mesothelial monolayers for 7 days to determine their invasive potential. Photos show representative examples of patient samples 2, 3, 4, and 5 at t = 0, and days 1, 4, and 7. Arrows delineate the perimeters of disaggregating or invading spheroids. Magnification = 100×.