Ascites spheroids demonstrate variable disaggregation on ECM components. Patient ascites spheroids were photographed on various ECM components at t = 0 and 24 hours later. Ascites spheroids demonstrated variable migration in response to ECM components. The top panels show outgrowth of patient 9 spheroids on fibronectin, which is representative of the extent of disaggregation for most patient spheroid samples. The middle panels show a spheroid from patient 4 totally disaggregating on type I collagen. The bottom panels show two spheroids, indicated by arrows, from patient 3 on type I collagen, with one spheroid completely disaggregated (left) and one unaltered (right). Magnification = 400×.