Figure 4

HLA-A2 allele stabilization with peptides derived from the E6 and E7 proteins. The lymphoblastic T2 cell line, which express empty HLA-A*0201 molecules on their cell surface, was loaded with different concentrations of the peptides: TLGIVCPIC and YMLDLQPETT derived from the E7 HPV-16 protein and KLPDLCTEL of the E6 HPV-18 protein. The peptide GILGFVFTL derived from the M Influenza-A protein, was used as positive control for binding assay. After incubation overnight with the peptides, conformational expression of the HLA-A2 allele was determined by flow cytometry using the PA2.1 MAb. MFI, mean fluorescence intensity.