Figure 3

Methylation and acetylation of HLA Class-I genes. A. HLA-A, -B anc -C gene promoters were analyzed by MSP in the cervical cancer cell lines. The control for unmethylated reaction is modified DNA from normal lymphocytes and for methylated is the same DNA but methylated in vitro. B. Chromatin immunoprecipitation assay with an anti-H4 acetylated antibody in Caski cells. Treatment with H/VA but no IFN-gamma led to H4 hyperacetylation. C. RT-PCR analysis of HLA-A, -B and -C, after treatment with H, H/VA and H/VA in SW480 colon carcinoma cell line and D. MSP (only with methylated primers shown) of the HLA-B promoter in SW480 cells indicating the lack of demethylation by H and 5-aza-2'-deoxycytidine.