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Figure 7 | Journal of Translational Medicine

Figure 7

From: Characterization of highly frequent epitope-specific CD45RA+/CCR7+/- T lymphocyte responses against p53-binding domains of the human polyomavirus BK large tumor antigen in HLA-A*0201+ BKV-seropositive donors

Figure 7

BKV LTag 579 specific CD8+ T cells belong to the CD45RA+ subpopulation. CD8+ T cells from HLA-A*0201+ BKV LTag seropositive donors were separated into CD45RA+ and CD45RA- fractions prior to stimulation with HLA-A*0201 restricted melanoma associated antigen MelanA/Mart-126–35 or BKV LTag 579 peptide. After 3-weekly restimulations in the presence of peptide-loaded autologous mature DCs, cytotoxic activity was tested by 51Cr release assays, using as targets T2 cells following pre-incubation in the presence of specific or control peptides. The phenotypes of CD8+ cells stained by MelanA/Mart-126–35 or BKV LTag 579 specific HLA-A*0201 multimers were also evaluated (panel A). Peptide specific IFN-γ production elicited by CD45RA+ and CD45RA- CD8+ T cell subpopulations is reported on panel B.

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Journal of Translational Medicine

ISSN: 1479-5876

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