Figure 5

Localization of pluripotency markers. Alkaline phosphatase (AP) activity (left), Oct-4 (center), and TROMA-1 (right) expression as measured in expanded and hatched blastocysts (top row, a-c), mouse embryonic fibroblasts (middle row, d-f) and embryonic stem cell colonies (bottom row, g-i) by immunofluorescent analysis. In left column, red signals indicate positive AP activity (a, g). In center, red signals identify positive Oct-4 expression (b, h) while the right column TROMA-1 expression is shown as green signals (c, hatched blastocyst). DAPI was used for nuclear DNA counterstaining as indicated by blue signals. Scale = 30 μm.