Figure 5

Reproducibility of whole blood flow cytometric enumeration of DCs. Scatter plots of independent duplicate analyses of each individual fresh whole blood sample are presented for the CD14 containing lineage cocktail, Panel A, and the CD66acde containing lineage cocktail, Panel B. There is a high degree of concordance between replicate analyses including data from all gating strategies, Panel A, (CD14 containing cocktail) with the best fit line constrained by passing through the origin and Pearson's R being y = 1.036x R = 0.9968. Panel B depicts the identical data for the CD66acde containing cocktail, y = 0.9417x R = 0.9740. Data restricted to Gate C yields a similar degree of concordance between replicate analyses with the best fit line constrained by passing through the origin and Pearson's R being y = 1.012x R = 0.9367 for CD14 lineage cocktail analyses, Panel C, and for analyses using the CD66acde containing cocktail being y = 0.9463x R = 0.9703, Panel D.