Figure 3
Effect of alternate gating strategies on DC enumeration by lineage cocktail. Box and whisker plots of compiled data for percentage DCs in normal and patient samples using the three alternate gating strategies. Normal samples evaluated using the CD14 containing lineage cocktail are depicted in Panel A and evaluated using the CD66 containing lineage cocktail depicted in Panel B, using the designated gates on the "X" axis. Patient samples evaluated using the CD14 containing lineage cocktail are depicted in Panel C and evaluated using the CD66 containing lineage cocktail depicted in Panel D, using the designated gates on the "X" axis. Within each sample set the differences between analyses using each of the three gates are statistically significant at p values ≤ 0.0312. Direct comparison of enumerated values for putative DCs in both "Normal and "Patient" baseline samples as detected using each lineage cocktail with Gate B, Panel E, and with Gate C, Panel F, reveals consistent and statistically significant differences, as noted, between determinations using the different cocktails.