Figure 1

Alternate "gates" for flow cytometric enumeration of circulating dendritic cells. Representative dot plots of two color flow cytometric evaluations from one of the six anonymous "normal" donor samples. All plots depict log fluorescence of FL-1 (Lineage – FITC) on the "Y axis" and FL-2 on the "X" axis (MHC II – PE) with collection of a minimum of 50,000 events. The left hand column depicts "Gate A", classic quadrant gates. The middle column depicts the least restrictive of the arbitrarily set MHC class II high expression subset "Gate B". The right hand column depicts the most restrictive MHC class II high expressing population, "Gate C". The first row represents isotype control analyses and gate settings that have 0.09 %, 0.00%, 0.00 % ≥ background, respectively.