Figure 2
From: The changes of CD4+CD25+/CD4+ proportion in spleen of tumor-bearing BALB/c mice
The relationship between tumor sizes and the CD4+CD25+/CD4+ proportions in peripheral blood or spleen lymphocytes in tumor bearing mice. (A) 1 × 105 to 1 × 107 C26 colon carcinoma cells were inoculated subcutaneously at right axilla of BALB/c mice (n = 12). 20 days later, after tumor sizes were measured, the mice were sacrificed and peripheral blood lymphocytes (○) and spleen (■) lymphocytes were stained with anti-mouse CD4 and CD25 antibodies. x-axis represents the diameters of tumors; y-axis represents the proportion of CD4+CD25+/CD4+. The absolute total lymphocyte counts were 9.85 ± 2.34 (× 109/L) in peripheral blood and 2.37 ± 0.77 (× 108) in spleen. The representative figures of CD4+CD25+ subset in peripheral blood (B) or spleen (C) lymphocytes from tumor bearing mice were also shown.