Figure 3
From: Protein kinase activity is associated with CD63 in melanoma cells
Chelerythrine inhibited cell adhesion to immobilized CD63 mAb. Plates were coated with 0.1 ug/ml of the CD63 mAb AHN-16 or the anti-MCSP mAb 9.2.27 as described in Materials and Methods. Cells were preincubated with various concentrations of chelerythrine for 20 min before adding the cells to the plates for the cell adhesion assays. Cell adhesion assays were performed for 30 min. Results are shown as percent adhesion +/- SEM. A duplicate experiment gave similar results.